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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01317
Strain Name∆H3/H4, major, gl KO 7(19)
SpeciesT. thermophila
Genetic Descriptionhht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2];hhf1[neo1]/hhf1[neo1]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?)
MAC Knockins/outs
MIC Knockins/outsNeo2 into HHT1 and KO HHT2/HHF2 gene and its intergenic region; KO of HHF1
Gene of InterestHHT + HHF
OriginYifan Liu
PublicationsCui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.

Liu Y, Song X, Gorovsky MA, Karrer KM. 2005. Elimination of foreign DNA during somatic differentiation in tetrahymena thermophila shows position effect and is dosage dependent. Eukaryot Cell 4(2):421-31.
CommentsThis is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2. KO with Neo cassette. HHF1 KO. It’s been tested to cross with ∆H3/H4 major,4(1) and the progeny died. Somatic rescue successful

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01317
Requires MTA?No
Academic cost $45