Stock ID | SD01254 |
Strain Name | SB2441H |
AKA | Precursor for meiotic segregant panel 2B |
Species | T. thermophila |
Genetic Description | chx1-1/chx1-1; pmr1-1/pmr1-1; MAT3/MAT3 (CHX1[C3]; cy-s, ?) |
MAC Knockins/outs | |
MIC Knockins/outs | |
Gene of Interest | |
Origin | B x C3 F1 progeny were induced to undergo meiosis by crossing them to A*. This clone is a round I heterokaryon strain. To generate the panel member it must be crossed in mass to A* for two rounds. True progeny are selected for by resistance to cycloheximide. |
Publications | Lynch TJ, Brickner J, Nakano KJ, Orias E. 1995. Genetic map of randomly amplified DNA polymorphisms closely linked to the mating type locus of tetrahymena thermophila. Genetics 141(4):1315-25. Brickner JH, Lynch TJ, Zeilinger D, Orias E. 1996. Identification, mapping and linkage analysis of randomly amplified DNA polymorphisms in tetrahymena thermophila. Genetics 143(2):811-21. |
Comments | This strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, pm-r, mat3. Resistance to 6-methylpurine was not scored for these panel members. Submitted by Eduardo Orias, University of California, Santa Barbara |
Research Resource Identifier | TSC_SD01254 |
Requires MTA? | No |
Academic cost | $45 |