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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD03327
Strain NameBTU2-mCherry
AKA
SpeciesT. thermophila
Genetic Description
MAC Knockins/outs
MIC Knockins/outs
Gene of Interest
Originfrom Jacek Gaertig
Publications
CommentsThe BTU2 locus was modified in the CU428 strain background by addition of a sequence encoding mCherry. The resulting beta-tubulin protein has a c-terminal mCherry tag. The protein is expressed at its own locus. There is a linked neo4 cassette that confers resistance to paromomycin (100 ug/ml) in the presence of cadmium chloride (2.5 ug/ml). These cells may be resistant to paromomycin 100 ugml or higher without added cadmium. The strain is believed to be assorted in the macronucleus but this has not confirmed using molecular means. If the expression level decreases these cells need to be grown at the increasing concentrations of paromomycin to accumulate the tagged allele.
Research Resource IdentifierTSC_SD03327
Requires MTA?No
Academic cost $45