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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD03309
Strain NameCU428/fsNEO
AKACU428/crNEO
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (WT; WT, VII)
MAC Knockins/outsRPL29::frame shift NEO
MIC Knockins/outs
Gene of Interest
OriginCU428 with NEO coding sequence with two frameshift mutations.
PublicationsStemm-Wolf AJ, Meehl JB, Winey M. 2013. Sfr13, a member of a large family of asymmetrically localized Sfi1-repeat proteins, is important for basal body separation and stability in tetrahymena thermophila. J Cell Sci 126(Pt 7):1659-71.
CommentsNEO coding sequence with two frameshift mutations in RPL29 vector (conferring resistance to Cyclohexamide, but remaining sensitive to paromomycin). The intention is this will provide the OLD MAC with a (defective) copy of the resistance gene, preventing its elimination in the NEW MAC after MIC transformation.

From the Winey Lab at the University of Colorado Boulder.
Research Resource IdentifierTSC_SD03309
Requires MTA?No
Academic cost $45