Stock ID | SD03307 |
Strain Name | B2086/fsNEO |
AKA | B2086/crNEO |
Species | T. thermophila |
Genetic Description | WT (WT; WT, ?) |
MAC Knockins/outs | RPL29::frame shift NEO |
MIC Knockins/outs | |
Gene of Interest | |
Origin | B2086 with NEO coding sequence with two frameshift mutations. |
Publications | Stemm-Wolf AJ, Meehl JB, Winey M. 2013. Sfr13, a member of a large family of asymmetrically localized Sfi1-repeat proteins, is important for basal body separation and stability in tetrahymena thermophila. J Cell Sci 126(Pt 7):1659-71. |
Comments | NEO coding sequence with two frameshift mutations in RPL29 vector (conferring resistance to Cyclohexamide, but remaining sensitive to paromomycin). The intention is this will provide the OLD MAC with a (defective) copy of the resistance gene, preventing its elimination in the NEW MAC after MIC transformation. From the Winey Lab at the University of Colorado Boulder. |
Research Resource Identifier | TSC_SD03307 |
Requires MTA? | No |
Academic cost | $45 |