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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD03307
Strain NameB2086/fsNEO
AKAB2086/crNEO
SpeciesT. thermophila
Genetic DescriptionWT (WT; WT, ?)
MAC Knockins/outsRPL29::frame shift NEO
MIC Knockins/outs
Gene of Interest
OriginB2086 with NEO coding sequence with two frameshift mutations.
PublicationsStemm-Wolf AJ, Meehl JB, Winey M. 2013. Sfr13, a member of a large family of asymmetrically localized Sfi1-repeat proteins, is important for basal body separation and stability in tetrahymena thermophila. J Cell Sci 126(Pt 7):1659-71.
CommentsNEO coding sequence with two frameshift mutations in RPL29 vector (conferring resistance to Cyclohexamide, but remaining sensitive to paromomycin). The intention is this will provide the OLD MAC with a (defective) copy of the resistance gene, preventing its elimination in the NEW MAC after MIC transformation.

From the Winey Lab at the University of Colorado Boulder.
Research Resource IdentifierTSC_SD03307
Requires MTA?No
Academic cost $45