Tetrahymena Stock Center| Stock ID | SD03306 |
| Strain Name | B2086/fsCodopt |
| AKA | B2086/crCodopt |
| Species | T. thermophila |
| Genetic Description | WT (WT; WT, II) |
| MAC Knockins/outs | RPL29::frame shift codon optimized NEO |
| MIC Knockins/outs | |
| Gene of Interest | |
| Origin | B2086 with codon optimized NEO coding sequence with two frameshift mutations. |
| Publications | |
| Comments | Codon optimized NEO coding sequence with two frameshift mutations in RPL29 vector (conferring resistance to Cyclohexamide, but remaining sensitive to paromomycin SELECT WITH 200ug/mL Pm). The intention is this will provide the OLD MAC with a (defective) copy of the resistance gene, preventing its elimination in the NEW MAC after MIC transformation. From the Winey Lab at the University of Colorado Boulder. |
| Research Resource Identifier | TSC_SD03306 |
| Requires MTA? | No |
| Academic cost | $45 |
