Stock ID | SD03297 |
Strain Name | UCB304 |
AKA | sfr13 KO/rescue22 |
Species | T. thermophila |
Genetic Description | sfr13-1[∆::NEO2] (sfr13-1[∆::NEO2]::SFR13[5'::BSR]::SFR13 3'; WT number of basal bodies (fewer cortical rows when analyzed)., ?) |
MAC Knockins/outs | SFR13 rescue in two parts (see comments) |
MIC Knockins/outs | cen2-1[∆::NEO2] |
Gene of Interest | SFR13 (TTHERM_00522830) |
Origin | UCB301 X UCB302 |
Publications | Stemm-Wolf AJ, Meehl JB, Winey M. 2013. Sfr13, a member of a large family of asymmetrically localized Sfi1-repeat proteins, is important for basal body separation and stability in tetrahymena thermophila. J Cell Sci 126(Pt 7):1659-71. |
Comments | sfr13 complete null rescue. First a 5' part of the gene was introduced into the sfr13 locus with BSR selection. Second, the remainder of the gene was introduced to the KO locus with the 5' BSR insertion, and rescue of the small cell size phenotype was selected for. From the Winey Lab at the University of Colorado Boulder. |
Research Resource Identifier | TSC_SD03297 |
Requires MTA? | No |
Academic cost | $45 |