Tetrahymena Stock Center| Stock ID | SD02871 |
| Strain Name | wt (ATU) (complete replacement) |
| AKA | |
| Species | T. thermophila |
| Genetic Description | atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[PvuII+HaeIII but WT except for sites].; pm-r) |
| MAC Knockins/outs | ATU1 with PvuII and HaeIII sites |
| MIC Knockins/outs | neo replaces ATU1 coding |
| Gene of Interest | ATU1 (TTHERM_00558620) |
| Origin | Bing Hai |
| Publications | Hai B and Gorovsky MA. 1997. Germ-line knockout heterokaryons of an essential alpha-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in tetrahymena thermophila. Proc Natl Acad Sci U S A 94(4):1310-5. Xia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6. |
| Comments | It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pTUB100E3-PvuII (linearized). have been assorted to pms. Its ATU gene is wt but marked with Hae III and Pvu II sites. Complete replacement. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
| Research Resource Identifier | TSC_SD02871 |
| Requires MTA? | No |
| Academic cost | $45 |
