Tetrahymena Stock Center| Stock ID | SD02703 |
| Strain Name | NNN-12-G |
| AKA | |
| Species | T. thermophila |
| Genetic Description | mpr1-1/mpr1-1 (ngoA[5’neo3, ∆::ngoA promoter 104bp,GFP]; VII) |
| MAC Knockins/outs | GFP into NgoA locus with 104 bp of NgoA promoter. and neo3 upstream |
| MIC Knockins/outs | |
| Gene of Interest | ngoA (TTHERM_00655890) |
| Origin | |
| Publications | Dou Y, Song X, Liu Y, Gorovsky MA. 2005. The H1 phosphorylation state regulates expression of CDC2 and other genes in response to starvation in tetrahymena thermophila. Mol Cell Biol 25(10):3914-22 |
| Comments | XS 35 ngoA 5’-neo3-ngoA ch2p12-GFP-ngoA3’ Somatic transformant replacing WT ngoA gene with GFP coding driven by ngoA 5’ flanking 104 bp. Neo3 cassette is upstream of the 104 bp. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
| Research Resource Identifier | TSC_SD02703 |
| Requires MTA? | No |
| Academic cost | $45 |
