Skip to main content


Follow @TetrahymenaSC

We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD02701
Strain NameMTT-HTAY-flag-MTT (XS76-31-20)
AKA
SpeciesT. thermophila
Genetic Description(MTT1/mtt1[[5’neo2;∆(::HTAY123-388)Flagc]; ?)
MAC Knockins/outsFlag tagged 2/3 c-terminus of HTAY into MTT1 locus
MIC Knockins/outs
Gene of InterestHTAY (TTHERM_01079200)
Origin
Publications
Comments(XS 76-31-20) Somatic transformant obtained by shooting CU428 X B2086 exconjugant cells. HTA.Y fused with Flag tag was used to replace MTT1 coding. Neo2 cassette is the selectable marker. Not complete.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02701
Requires MTA?No
Academic cost $45