Stock ID | SD02639 |
Strain Name | H3.3R |
AKA | |
Species | T. thermophila |
Genetic Description | hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (HHF2HHT.3; pm-r, ?) |
MAC Knockins/outs | GFP H3.3 with wt HHF2 into HHT2HHF2 loccus |
MIC Knockins/outs | Neo KO of all major HHT genes and HHF genes |
Gene of Interest | HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170), HHT2 (TTHERM_00189180) |
Origin | |
Publications | Cui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30. |
Comments | Germline heteokaryon Major H3 and H4 genes knocked out with neo cassette.HHT.3 inyo HHT2 locus with HHF2. HHT3 wt is still there. can be mated with B2086. From the Gorovsky lab, University of Rochester. |
Research Resource Identifier | TSC_SD02639 |
Requires MTA? | No |
Academic cost | $45 |