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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD02614
Strain Nameg∆∆-1
AKA
SpeciesT. thermophila
Genetic Descriptionhht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1];hht2[GFPc]/hht2[GFPc] (HHT2-GFP, ∆HHT3, ∆HHT4; pm-r,bsr-r, ?)
MAC Knockins/outsNeo KO of HHT3, Bsr KO of HHT4, GFP tagged HHT2
MIC Knockins/outsNeo KO of HHT3, Bsr KO of HHT4, GFP tagged HHT2
Gene of InterestHHT2 (TTHERM_00189180), HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Origin
PublicationsCui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
CommentsH3.2-GFP heterokaryon is crossed with ∆3N4B heterokaryon, the heterozygotes then go through Round I and the * side and non* side are then mated to obtain the homozygous homokaryons.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02614
Requires MTA?No
Academic cost $45