Stock ID | SD02474 |
Strain Name | K286EHA#7 |
AKA | |
Species | T. thermophila |
Genetic Description | gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU1, K286E), HAc]; pm-r, ?) |
MAC Knockins/outs | GTU1 with mutation K286E and HA tag at c-teminus into the MTT1 locus, partial replacement. |
MIC Knockins/outs | Neo2 into GTU1 locus |
Gene of Interest | GTU1 (TTHERM_00079520) |
Origin | |
Publications | Shang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206. Shang Y, Tsao CC, Gorovsky MA. 2005. Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. J Cell Biol 171(6):1035-44. |
Comments | pK268EHA rescued GTUKO 5x6 progeny having mutation @ K268E in GTU1-1 gene, also one HA tag@c-terminus; driven by MTT1 promoter. Needs Cd to induce. Not single celled yet. They were ~2 weeks old. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
Research Resource Identifier | TSC_SD02474 |
Requires MTA? | No |
Academic cost | $45 |