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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD02320
Strain NameH4.2 K15R
AKA
SpeciesT. thermophila
Genetic Descriptionhhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2] (hht1[∆::neo2]; hht1[∆::neo2]; HHT2; hhf2[K15R]; pm-r, ?)
MAC Knockins/outsWT HHF2 and mutated HHT2 with K9Q
MIC Knockins/outsNeo KO of all major HHT genes and HHF genes
Gene of InterestHHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
OriginYifan Liu
PublicationsLiu Y, Song X, Gorovsky MA, Karrer KM. 2005. Elimination of foreign DNA during somatic differentiation in tetrahymena thermophila shows position effect and is dosage dependent. Eukaryot Cell 4(2):421-31.

Liu Y. 2003. Genetic studies of RNA-mediated DNA elimination in the developing macronuclei of tetrahymena thermophila. University of Rochester.
CommentsGermline heteokaryon Major H3 and H4 KO stains were mated and rescued with wt HHT2 gene/HHF2 gene with K15R mutation.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02320
Requires MTA?No
Academic cost $45