Tetrahymena Stock Center| Stock ID | SD02195 |
| Strain Name | TYAHA@MTT1 locus s.c. line #6 |
| AKA | |
| Species | T. thermophila |
| Genetic Description | gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, HAc(::GTU1, T270A, Y272A)]; pm-r, ?) |
| MAC Knockins/outs | GTU1 with mutations T270A, Y272A anc c-terminus HA tag into the MTT1 locus, partial replacement. |
| MIC Knockins/outs | Neo2 into GTU1 locus |
| Gene of Interest | GTU1 (TTHERM_00079520) |
| Origin | Yuhua Shang |
| Publications | Shang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206. Shang Y, Tsao CC, Gorovsky MA. 2005. Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. J Cell Biol 171(6):1035-44. |
| Comments | pR285D291 rescued GTUKO 5x6 progeny having mutation @ T270,Y272-AA in GTU1-1 gene, driven by MTT1 promoter. Needs Cd to induce. Has HA tag at c-terminus. #3 can be crossed with #6. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
| Research Resource Identifier | TSC_SD02195 |
| Requires MTA? | No |
| Academic cost | $45 |
