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Stock IDSD01898
Strain NameS-H3.2+GFP/H3.3-422
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (HHT3?/htt3[5’neo2, ∆, (::HHT2,GFPc)]; pm-r, VII)
MAC Knockins/outsHHT2 with GFP c-terminal tag replaces the HHT3 coding region
MIC Knockins/outs
Gene of InterestHHT2 (TTHERM_00189180), HHT3 (TTHERM_00016170)
OriginBowen Cui
PublicationsCui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
Commentssomatic replace H3.3 coding with H3.2+GFP, neo 2 cassette in 5’ flank of H3.3. Not sure if complete replacement.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01898
Requires MTA?No
Academic cost $35