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Stock IDSD01892
Strain NameHA-hv2 / B2086
AKA
SpeciesT. thermophila
Genetic Description(?HHT3/hht3[3’neo2, HAn]; pm-r, II)
MAC Knockins/outsN-terminal HA tag of HHT3, not sure if complete replacement
MIC Knockins/outs
Gene of InterestHTT3 (TTHERM_00016170)
OriginLanlan Yu
PublicationsYu L and Gorovsky MA. 1997. Constitutive expression, not a particular primary sequence, is the important feature of the H3 replacement variant hv2 in tetrahymena thermophila. Mol Cell Biol 17(11):6303-10.

Cui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
CommentsHA-tagged hv2 mac transformant. Checked by western blot. Hahv2 is expressed. This strain can be used to check HA-hv3 localization in conjugated cells by mating with HA-hv2/CU428.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01892
Requires MTA?No
Academic cost $35