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Stock IDSD01884
Strain Name∆HTB2/HTB1 A8C K4R
AKA
SpeciesT. thermophila
Genetic Descriptionhtb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (HTB1/htb1[A8C, K4R]; htb2 [∆::neo2] assorted to pm-s so must have lost neo2; HTB1with A8C, K4R, ∆htb2, pm-s, cy-s, mp-r,, ?)
MAC Knockins/outsHTB1 with A8C, K4R mutation
MIC Knockins/outsneo2 into coding of HTB1 and HTB2
Gene of InterestHTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
OriginNing Liu
PublicationsWang Z. 2007. Functional studies of histone ubiquitylation in tetrahymena thermophila. University of Rochester.

Wang Z, Cui B, Gorovsky MA. 2009. Histone H2B ubiquitylation is not required for histone H3 methylation at lysine 4 in tetrahymena. J Biol Chem 284(50):34870-9.
CommentsNew batch, rescue of progeny h∆∆HTB86 B4A6 and D2A2 with HTB1 with A8C, K4R mutation

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01884
Requires MTA?No
Academic cost $35