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Stock IDSD01857
Strain NameH3.4-Flag-4
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (?HHT4/hht4[∆, HHT3, 5’neo2,FLAGc]; pm-r, VII)
MAC Knockins/outsN-terminalFlag tagged HHT3 put into HHT4 locus, not sure if complete replacement
MIC Knockins/outs
Gene of InterestHTT4 (TTHERM_00016200), HHT3 (TTHERM_00016170)
OriginBowen Cui
PublicationsYu L and Gorovsky MA. 1997. Constitutive expression, not a particular primary sequence, is the important feature of the H3 replacement variant hv2 in tetrahymena thermophila. Mol Cell Biol 17(11):6303-10.

Cui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
CommentsMac transformant. Somatic replace HHT4 gene with HHT3-Flag sequence. Neo2 in 5’ flanking of HHT3

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01857
Requires MTA?No
Academic cost $35