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Stock IDSD01834
Strain NameAAA2D9
SpeciesT. thermophila
Genetic Descriptionbtu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-?[E431D,E432D,E433D,E435D,E437D,E438D,E439D,E440D,E442D]; ATU1/atu1-1[duplicate ATU1 tail--AAA2]; pm-r, Original BTU2 is replaced by bsr1, BTU1 coding replaced by neo1, then transformed with BTU1 with (E431-442D) and Chimeric ATU1 gene, AAA2 (duplicate ATU C-tail))
MAC Knockins/outsBTU2 replaced by bsr. BTU1 partially replaced by neo1, some replaced by BTU1-D9 with (E431-442D). Some ATU1 replaced by ATU1 with duplicate C-tail
MIC Knockins/outsknockout of btu1 with neo
knockout of btu2 with bsr
Gene of InterestBTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580), ATU1 (TTHERM_00558620)
OriginJianming Duan
PublicationsDuan J and Gorovsky MA. 2002. Both carboxy-terminal tails of alpha- and beta-tubulin are essential, but either one will suffice. Curr Biol 12(4):313-6.

Xia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6.

Thazhath R, Jerka-Dziadosz M, Duan J, Wloga D, Gorovsky MA, Frankel J, Gaertig J. 2004. Cell context-specific effects of the beta-tubulin glycylation domain on assembly and size of microtubular organelles. Mol Biol Cell 15(9):4136-47.
CommentsProgeny of DB6B x DB2A, co- transformed by constructs BTU1-D9 with (E431-442D) and construct of chimeric ATU1 gene, AAA2 duplicate ATU C-tail). See publications

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01834
Requires MTA?No
Academic cost $35