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Stock IDSD01828
Strain NameA438-1
AKA
SpeciesT. thermophila
Genetic Descriptionatu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E438A]; pm-r, mp-?)
MAC Knockins/outsATU1 with E438A or neo replacing coding of some ATU1 genes
MIC Knockins/outsneo replaces ATU1 coding
Gene of InterestATU1 (TTHERM_00558620)
OriginBing Hai
PublicationsHai B and Gorovsky MA. 1997. Germ-line knockout heterokaryons of an essential alpha-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in tetrahymena thermophila. Proc Natl Acad Sci U S A 94(4):1310-5.

Xia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6.
CommentsIt is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pTUB100E3-PvuII (linearized). Its mac ATU1 gene is marked with Hae III and Pvu II sites and has E438A

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD01828
Requires MTA?No
Academic cost $35