Tetrahymena Stock Center| Stock ID | SD01813 |
| Strain Name | BMCG-hy3-63 |
| AKA | |
| Species | T. thermophila |
| Genetic Description | ∆hht1 & ∆hht2 ∆hhf1 ∆hhf2 hht1[∆,neo2]/hht1[∆,neo2]; hhf1[∆,neo]/hhf1[∆,neo]; hhf2,hht2[∆, neo2]/ hhf2,hht2[∆, neo2] (hht1[∆,neo2]/hhf1[∆,neo]/hhf2,hht2[∆, neo2]/ chimeric H3[H3(1-75) H3.3(76-90) H3(91-135)] MTT1/mtt1-?[5’ neo2,∆,(::CNA1), GFPc]; pm-r, bsr-1, hybrid H3 replaces major H3. In MTT1 locus,GFP tagged CNA1) |
| MAC Knockins/outs | hybrid H3 replaces major H3. In MTT1 locus,GFP tagged CNA1 |
| MIC Knockins/outs | ∆hht1 ; ∆hht2 ∆hhf1; ∆hhf2 |
| Gene of Interest | CNA1 (TTHERM_00146340), HHT2 (TTHERM_00189180), HHT3 (TTHERM_00016170) |
| Origin | Bowen Cui |
| Publications | Liu Y, Song X, Gorovsky MA, Karrer KM. 2005. Elimination of foreign DNA during somatic differentiation in tetrahymena thermophila shows position effect and is dosage dependent. Eukaryot Cell 4(2):421-31. |
| Comments | major h3, h4 germline KO cells were rescued with hy3. Hybrid H3 contruct with H3(1-75) H3.3 (76-90) H3 (91-135), then transformed with Bsr-mtt1-cna1-gfp construct. CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cas. Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
| Research Resource Identifier | TSC_SD01813 |
| Requires MTA? | No |
| Academic cost | $45 |
