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Stock IDSD01786
Strain Name∆HTAY pXS83-23
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (HTAY?/htay[∆::neo3]; MTT1/mtt1[5’neo2, ∆, 2HAn(::HTAY)]; pm-r, VII)
MAC Knockins/outsNeo3 KO of HTAY(may be incomplete) and HTAY with two HA tags on the N-terminus inserted into MTT1 locus with neo2 cassete in 5’ flanking region
MIC Knockins/outs
Gene of InterestH2A.Y (TTHERM_01079200)
OriginXiaoyuan Song
PublicationsSong X, Bowen J, Miao W, Liu Y, Gorovsky MA. 2012. The nonhistone, N-terminal tail of an essential, chimeric H2A variant regulates mitotic H3-S10 dephosphorylation. Genes Dev 26(6):615-29.
CommentsTransformed pXS83 (MTT-neo2-2HA-HTAY-MTT) into HTA.Y real somatic knockout strain in MTT1 locus.Selected in the presence of Cd +Pm. PCR shows very faint band of WT HTA.Y and WT MTT1 copy left (might come from mic).

Grow cells 0.5 ug/ml Cd and .5 mg/ml pm and increase that up to 6 mg/ml with passage.

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD01786
Requires MTA?No
Academic cost $35