Stock ID | SD01786 |
Strain Name | ∆HTAY pXS83-23 |
AKA | |
Species | T. thermophila |
Genetic Description | mpr1-1/mpr1-1 (HTAY?/htay[∆::neo3]; MTT1/mtt1[5’neo2, ∆, 2HAn(::HTAY)]; pm-r, VII) |
MAC Knockins/outs | Neo3 KO of HTAY(may be incomplete) and HTAY with two HA tags on the N-terminus inserted into MTT1 locus with neo2 cassete in 5’ flanking region |
MIC Knockins/outs | |
Gene of Interest | H2A.Y (TTHERM_01079200) |
Origin | Xiaoyuan Song |
Publications | Song X, Bowen J, Miao W, Liu Y, Gorovsky MA. 2012. The nonhistone, N-terminal tail of an essential, chimeric H2A variant regulates mitotic H3-S10 dephosphorylation. Genes Dev 26(6):615-29. |
Comments | Transformed pXS83 (MTT-neo2-2HA-HTAY-MTT) into HTA.Y real somatic knockout strain in MTT1 locus.Selected in the presence of Cd +Pm. PCR shows very faint band of WT HTA.Y and WT MTT1 copy left (might come from mic). Grow cells 0.5 ug/ml Cd and .5 mg/ml pm and increase that up to 6 mg/ml with passage. From the Gorovsky lab, University of Rochester. |
Research Resource Identifier | TSC_SD01786 |
Requires MTA? | No |
Academic cost | $45 |