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Stock IDSD01780
Strain NameS-MTT-H3.3-G-IN3
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (MTT1?/mtt1[5’neo2, ∆, (::HHT3,GFPc)]; pm-r, VII)
MAC Knockins/outsHHT3 with GFP c-terminal tag replaces the MTT1 coding region
MIC Knockins/outs
Gene of InterestHHT3 (TTHERM_00016170)
OriginBowen Cui
PublicationsCui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
CommentsSomatic replace MTT1 coding with H3.3+GFP, neo 2 cassette in 5’ flank. Not sure if complete replacement. Makes hv2 histone variant protein.

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD01780
Requires MTA?No
Academic cost $35