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Stock IDSD01778
Strain Nameg∆∆-3
AKA
SpeciesT. thermophila
Genetic Descriptionhht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1];hht2[GFPc]/hht2[GFPc] (HHT2-GFP,∆HHT3,∆HHT4; pm-r,bsr-r, ?)
MAC Knockins/outsNeo KO of HHT3, Bsr KO of HHT4, GFP tagged HHT2
MIC Knockins/outsNeo KO of HHT3, Bsr KO of HHT4, GFP tagged HHT2
Gene of InterestHHT2 (TTHERM_00189180), HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Origin
PublicationsCui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
CommentsH3.2-GFP homozygous heterokaryon is crossed with ∆3N4B heterokaryon, the heterozygotes then go through Round I and the * side and non* side are then mated to obtain the homozygous homokaryons.

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD01778
Requires MTA?No
Academic cost $35