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Stock IDSD01777
Strain NameMCG-B
AKA
SpeciesT. thermophila
Genetic DescriptionMTT1/MTT1 (MTT1/mtt1-?[5’ neo2,∆,(::CNA1), GFPc]; Mtt1p/Cna1p-GFPc, II)
MAC Knockins/outsGFP cterminal tagged CNA1 coding, inserted into MTT1 locus
MIC Knockins/outs
Gene of InterestCenpA
Origin
PublicationsCui B and Gorovsky MA. 2006. Centromeric histone H3 is essential for vegetative cell division and for DNA elimination during conjugation in tetrahymena thermophila. Mol Cell Biol 26(12):4499-510.
CommentsMTT-CenpA-GFP was transformed into B2086 at MTT1 locus somatially. Not completed - grow in 0.5 ug/mlCd++and 200ug/ml paromomycin.

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD01777
Requires MTA?No
Academic cost $35