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Stock IDSD01757
Strain NameVPS13A::GFP
AKATtVPS13A::GFPA5
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1; VPS13A/VPS13A (MPR1; VPS13A/vps13a-2::gfp; pm-r, mp-s, VII, VII)
MAC Knockins/outs
MIC Knockins/outs
Gene of InterestVacuolar Protein Sorting 13A gene
OriginMacronuclear transformant of strain CU428.2 with a construct containing GFP fused to the C-terminus of the TtVPS13A gene. The construct included neo gene under control of MTT1 promoter.
PublicationsSamaranayake H.S., Cowan A.E., Klobutcher L.A. 2011. Vacuolar protein sorting protein 13A, TtVPS13A, localizes to the tetrahymena thermophila phagosome membrane and is required for efficient phagocytosis. Eukaryotic Cell 10(9):1207-18.

Samaranayake HS. 2010. Assessing the role of tetrahymena thermophila vacuolar protein sorting 13A (TtVPS13A) in phagocytosis. University of Connecticut (PhD thesis).
CommentsDeposited line has been grown with high concentration of paromomycin (1 mg/ml) for phenotypic assortment, but it has not been subcloned. Gene has also been referred to as TtVPS13A. Stock has been maintained in SSP medium + 2 ug/ml CdCl2 + 1 mg/ml paromomycin.

Submitted by Lawrence Klobutcher, University of Connecticut Health Center
Research Resource IdentifierTSC_SD01757
Requires MTA?No
Academic cost $35