Tetrahymena Stock Center| Stock ID | SD01757 |
| Strain Name | VPS13A::GFP |
| AKA | TtVPS13A::GFPA5 |
| Species | T. thermophila |
| Genetic Description | mpr1-1/mpr1-1; VPS13A/VPS13A (MPR1; VPS13A/vps13a-2::gfp; pm-r, mp-s, VII, VII) |
| MAC Knockins/outs | |
| MIC Knockins/outs | |
| Gene of Interest | Vacuolar Protein Sorting 13A gene |
| Origin | Macronuclear transformant of strain CU428.2 with a construct containing GFP fused to the C-terminus of the TtVPS13A gene. The construct included neo gene under control of MTT1 promoter. |
| Publications | Samaranayake H.S., Cowan A.E., Klobutcher L.A. 2011. Vacuolar protein sorting protein 13A, TtVPS13A, localizes to the tetrahymena thermophila phagosome membrane and is required for efficient phagocytosis. Eukaryotic Cell 10(9):1207-18. Samaranayake HS. 2010. Assessing the role of tetrahymena thermophila vacuolar protein sorting 13A (TtVPS13A) in phagocytosis. University of Connecticut (PhD thesis). |
| Comments | Deposited line has been grown with high concentration of paromomycin (1 mg/ml) for phenotypic assortment, but it has not been subcloned. Gene has also been referred to as TtVPS13A. Stock has been maintained in SSP medium + 2 ug/ml CdCl2 + 1 mg/ml paromomycin. Submitted by Lawrence Klobutcher, University of Connecticut Health Center |
| Research Resource Identifier | TSC_SD01757 |
| Requires MTA? | No |
| Academic cost | $45 |
