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Stock IDSD01739
Strain NameVI004
AKA
SpeciesT. thermophila
Genetic Description(JMJ2/jmj2-1[Δ21-672(::neo4, KanR2)]; pm-r, II)
MAC Knockins/outsJMJ2 gene partially replaced by neo4-KanR2 cassette.
MIC Knockins/outsPAR1 gene partially replaced by neo4-KanR2 cassette.
Gene of InterestJMJ2 (TTHERM_00455500)
OriginSomatic biolistic transformation of B2086 strains using a neo4-KanR2 knockout cassette.
Publications
CommentsThe knockout also introduces a frameshift mutation. The knockout has been confirmed by paromomycin resistance, but macronuclear allele homozygosity has not been verified. The neo4-KanR2 knockout cassette was generated by cloning fragments of the pNeo4 plasmid's neo4 cassette and the pUC4K plasmid's KanR2 cassette. The KanR2 gene was used for E. coli transformation and is not a selectable marker in Tetrahymena.

Submitted by Robert Coyne, J. Craig Venter Institute
Research Resource IdentifierTSC_SD01739
Requires MTA?No
Academic cost $35