Tetrahymena Stock Center| Stock ID | SD01727 |
| Strain Name | RH179E1 |
| AKA | unsaturated fatty acid auxotroph |
| Species | T. thermophila |
| Genetic Description | chx1-1/chx1-1; mat2/mat2 (chx1-1; cy-r, ?) |
| MAC Knockins/outs | |
| MIC Knockins/outs | |
| Gene of Interest | |
| Origin | Robin Hill mutagenized a cycloheximide-resistant strain of Tetrahymena thermophila (inbred strain B), crossed these cells to C* and isolated short circuit conjugants. Screening found this strain which cannot grow in defined medium without fatty acids. |
| Publications | Hill RJ. 1980. Modulation of membrane fluidity in a fatty acid auxotrophe of tetrahymena thermophila. Biochim Biophys Acta 595(1):140-5. Hill RJ, Prescott DJ, Spence SL, Conner RL. 1983. Characterization of an unsaturated fatty acid auxotroph of tetrahymena thermophila. Biochimica Et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 754(2):150-8. Katz M, Conner RL, Baum MP, Orias E. 1987. Unidirectional co-stimulation by a non-mating strain of tetrahymena thermophila. Exp Cell Res 169(1):63-73. |
| Comments | Non-mater so the MIC genotype is presumptive. Submitted by Eileen Hamilton, UCSB |
| Research Resource Identifier | TSC_SD01727 |
| Requires MTA? | No |
| Academic cost | $45 |
