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Stock IDSD01727
Strain NameRH179E1
AKAunsaturated fatty acid auxotroph
SpeciesT. thermophila
Genetic Descriptionchx1-1/chx1-1; mat2/mat2 (chx1-1; cy-r, ?)
MAC Knockins/outs
MIC Knockins/outs
Gene of Interest
OriginRobin Hill mutagenized a cycloheximide-resistant strain of Tetrahymena thermophila (inbred strain B), crossed these cells to C* and isolated short circuit conjugants. Screening found this strain which cannot grow in defined medium without fatty acids.
PublicationsHill RJ. 1980. Modulation of membrane fluidity in a fatty acid auxotrophe of tetrahymena thermophila. Biochim Biophys Acta 595(1):140-5.

Hill RJ, Prescott DJ, Spence SL, Conner RL. 1983. Characterization of an unsaturated fatty acid auxotroph of tetrahymena thermophila. Biochimica Et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 754(2):150-8.

Katz M, Conner RL, Baum MP, Orias E. 1987. Unidirectional co-stimulation by a non-mating strain of tetrahymena thermophila. Exp Cell Res 169(1):63-73.
CommentsNon-mater so the MIC genotype is presumptive.

Submitted by Eileen Hamilton, UCSB
Research Resource IdentifierTSC_SD01727
Requires MTA?No
Academic cost $35