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Stock IDSD01628
Strain NameBI3840 IVa
AKApig
SpeciesT. thermophila
Genetic DescriptionNot applicable (pig?; chx1-1; pig, Cy-R, IV)
MAC Knockins/outs
MIC Knockins/outs
Gene of Interest
OriginOriginally isolated as a pigment-excreting mutant, "pig" (see Kaney and Knox, 1980); later discovered to be amicronucleate (Kaney and Speare, 1983). Stock culture obtained from A. Kaney in Feb. 1982.
PublicationsKaney AR and Knox GW. 1980. Production of melanin precursors by a mutant of tetrahymena thermophila. J Protozool 27(3):339-41.

Kaney AR and Speare VJ. 1983. An amicronucleate mutant of tetrahymena thermophila. Exp Cell Res 143(2):461-7.

Karrer K, Stein-Gavens S, Allitto BA. 1984. Micronucleus-specific DNA sequences in an amicronucleate mutant of tetrahymena. Dev Biol 105(1):121-9.

Kaney AR. 1985. A transmissible developmental block in tetrahymena thermophila. Exp Cell Res 157(2):315-21.

Frankel J and Nelsen EM. 1986. Intracellular pattern reversal in tetrahymena thermophila. II. transient expression of a janus phenocopy in balanced doublets. Dev Biol 114(1):72-86.
CommentsBI3840 cannot indefinitely maintain an introduced micronucleus (Kaney and Speare, 1983) and is developmentally defective (Kaney, 1985). Some DNA sequences that are limited to the micronucleus in wild-type cells are present in the macronucleus in BI3840 (Karrer et al., 1984). BI3840 has a tendency to produce homopolar doublets (Frankel & Nelsen, 1986). This stock probably corresponds to clone #1 in Frankel & Nelsen, (1986), isolated on 3/1/82.

Submitted by Joseph Frankel, University of Iowa
Research Resource IdentifierTSC_SD01628
Requires MTA?No
Academic cost $35