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Stock IDSD01567
Strain NameIA203
AKAjanA-1 IVd
SpeciesT. thermophila
Genetic DescriptionjanA-1/janA-1; eja1-1/eja1-1 (janA-1; eja1-1; janA, eja1, IV)
MAC Knockins/outs
MIC Knockins/outs
Gene of InterestJANA (janusA) (originally published as jan (janus); EJA (enhancer of janA)
OriginThe original janA clone was obtained by genetic rescue of a clone selected in 1976 by Bruns and Sanford (PNAS 75: 3355-3358) using nitrosoguanidine mutagenesis followed by short-circuit genomic exclusion. The derivation of IA203 included an outcross of the original janA clone to CU329, followed by two generations of outcrossing to B1975 III. IA 203 is one generation "downstream" from the foundation janA stock IA192 (unfortunately non-recoverable) and one generation "upstream" from IA220.
PublicationsFrankel J and Jenkins LM. 1979. A mutant of tetrahymena thermophila with a partial mirror-image duplication of cell surface pattern. II. nature of genic control. J Embryol Exp Morphol 49:203-27.

Frankel J, Jenkins LM, Bakowska J. 1984. Selective mirror-image reversal of ciliary patterns intetrahymena thermophila homozygous for ajanus mutation. Development Genes and Evolution 194(2):107-20.

Frankel J and Nelsen EM. 1987. Positional reorganization in compound janus cells of tetrahymena thermophila. Development 99(1):51-68.
CommentsConversion of the cortical pattern of the dorsal cell surface to a partial mirror-image of the ventral surface; inability to complete conjugation.

Submitted by Joseph Frankel, University of Iowa
Research Resource IdentifierTSC_SD01567
Requires MTA?No
Academic cost $35