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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD01536
Strain NameSB1514
AKAtyrF13
SpeciesT. thermophila
Genetic Descriptiongal1-1/gal1-1; tyrF13/tyrF13 (gal1-1; tyrF13; gal-r; tyr auxotroph, II)
MAC Knockins/outs
MIC Knockins/outs
Gene of Interest
OriginSB210 cells were exposed to nitrosoguanidine and crossed to CU329 cells. Self-fertilization (cytogamy) was induced. Self-fertilized progeny were selected for by exploiting the recessiveness of the 2-deoxygalactose-resistance mutation. The cloned progeny were then replicated to rich medium and defined medium at 38°C, and those that grew poorly or not at all in defined medium but well in complex medium, were then tested in defined medium + tyrosine.
PublicationsSanford YM and Orias E. 1981. Phenylketonuric tetrahymena: Phenylalanine hydroxylase mutants and other tyrosine auxotrophs. Proc Natl Acad Sci U S A 78(12):7614-8.
CommentsSubmitted by Eduardo Orias, University of California, Santa Barbara
Research Resource IdentifierTSC_SD01536
Requires MTA?No
Academic cost $45