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Stock IDSD01536
Strain NameSB1514
SpeciesT. thermophila
Genetic Descriptiongal1-1/gal1-1; tyrF13/tyrF13 (gal1-1; tyrF13; gal-r; tyr auxotroph, II)
MAC Knockins/outs
MIC Knockins/outs
Gene of Interest
OriginSB210 cells were exposed to nitrosoguanidine and crossed to CU329 cells. Self-fertilization (cytogamy) was induced. Self-fertilized progeny were selected for by exploiting the recessiveness of the 2-deoxygalactose-resistance mutation. The cloned progeny were then replicated to rich medium and defined medium at 38°C, and those that grew poorly or not at all in defined medium but well in complex medium, were then tested in defined medium + tyrosine.
PublicationsSanford YM and Orias E. 1981. Phenylketonuric tetrahymena: Phenylalanine hydroxylase mutants and other tyrosine auxotrophs. Proc Natl Acad Sci U S A 78(12):7614-8.
CommentsSubmitted by Eduardo Orias, University of California, Santa Barbara
Research Resource IdentifierTSC_SD01536
Requires MTA?No
Academic cost $35