Stock ID | SD01466 |
Strain Name | IA480 |
AKA | hpo1-4 II |
Species | T. thermophila |
Genetic Description | hpo1-4/hpo1-4 (hpo1-4; II) |
MAC Knockins/outs | |
MIC Knockins/outs | |
Gene of Interest | HPO1 (hypoangular) |
Origin | Enigmatic. It arose from a screen designed to detect revertants of janC1. Following nitrosoguanidine mutagenesis of a janC1 heterokaryon in 1994 and uniparental cytogamy, a mutant homozygote was outcrossed to B2086 VIb and then carried through genomic exclusion. The mutant progeny, including IA480, all expressed the hypoangular phenotype and showed no trace of the janus phenotype. |
Publications | None. Very similar to hpo1-1, described in Frankel J, Jenkins LM, Nelsen EM, Stoltzman CA. 1993. Hypoangular: A gene potentially involved in specifying positional information in a ciliate, tetrahymena thermophila. Dev Biol 160(2):333-54. |
Comments | A complex phenotype with contractile vacuole pores too close to the oral apparatus, and clockwise slippage of oral primordia. Similar to hpo1-1, though much less extensively studied. Detailed phenotypic analysis was carried out on the sister clone, IA481. Submitted by Joseph Frankel, University of Iowa |
Research Resource Identifier | TSC_SD01466 |
Requires MTA? | No |
Academic cost | $45 |