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Stock IDSD01460
Strain NameIA313
AKAbig1-1 II
SpeciesT. thermophila
Genetic Descriptionbig1-1/big1-1 (big1-1; II)
MAC Knockins/outs
MIC Knockins/outs
Gene of InterestBIG1 (big 1)
OriginGenomic exclusion progeny following 2 successive generations of outcrossing to B2079-X-II (with an intervening genomic exclusion cross) of a mutant clone resulting from nitrosoguanidine mutagenesis of IA264 in 1981 followed by cytogamy
PublicationsFrankel J, Jenkins LM, Bakowska J, Nelsen EM. 1984b. Mutational analysis of patterning of oral structures in tetrahymena. I. effects of increased size on organization. J Embryol Exp Morphol 82:41-66.
CommentsIncrease in size of cells, number of ciliary rows, and size of oral apparatus. Ciliary rows are disorganized, and 10-20% of oral apparatuses are abnormal, with extra membranelles common.

Highly pleiotropic. The disorganization of the ciliary rows was not mentioned in the brief published description, but is prominent. Expression unform at all temperatures tested.

Submitted by Joseph Frankel, University of Iowa
Research Resource IdentifierTSC_SD01460
Requires MTA?No
Academic cost $35