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Stock IDSD01459
Strain NameIA230
AKAcdaF-1 VI
SpeciesT. thermophila
Genetic DescriptioncdaF-1/cdaF-1 (cdaF-1; VI)
MAC Knockins/outs
MIC Knockins/outs
Gene of InterestCDAF (cell division arrest F) (originally published as mo12)
OriginGenomic exclusion progeny following an outcross to CU329 of an F2 that had been obtained following an earlier outcross to B1868 VII of an assortant derived from nitrosoguanidine mutagenesis of B1868 VI x VII in 1974.
PublicationsFrankel J, Jenkins LM, Doerder FP, Nelsen EM. 1976. Mutations affecting cell division in tetrahymena pyriformis. I. selection and genetic analysis. Genetics 83(3):489-506.

Frankel J, Nelsen EM, Jenkins LM. 1977. Mutations affecting cell division in tetrahymena pyriformis, syngen 1. II. phenotypes of single and double homozygotes. Dev Biol 58(2):255-75.
CommentsLate fission arrest with failure of subdivision of some ciliary rows (39°).

A clear-cut temperature-sensitive mutant. Penetrance is high but not 100% at 39° C.

The published phenotypic analysis was carried out on a homozygous (F2) clone of the previous sexual generation, which is now extinct.

Submitted by Joseph Frankel, University of Iowa
Research Resource IdentifierTSC_SD01459
Requires MTA?No
Academic cost $35