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Stock IDSD01458
Strain NameIA225
AKAcdaE-3 IIA
SpeciesT. thermophila
Genetic DescriptioncdaE-3/cdaE-3 (cdaE-3; II)
MAC Knockins/outs
MIC Knockins/outs
Gene of InterestCDAE (cell division arrest E)
OriginGenomic exclusion progeny following an outcross to B2079-X-VI of a mutant clone that resulted from nitrosoguanidine mutagenesis in 1979 of SB210 followed by cytogamy.
PublicationsNone. The allelic cdaE-1/cdaE-1 (IA137) was described in:
Frankel J, Jenkins LM, Doerder FP, Nelsen EM. 1976. Mutations affecting cell division in tetrahymena pyriformis. I. selection and genetic analysis. Genetics 83(3):489-506.

Frankel J, Nelsen EM, Jenkins LM. 1977. Mutations affecting cell division in tetrahymena pyriformis, syngen 1. II. phenotypes of single and double homozygotes. Dev Biol 58(2):255-75.
CommentsIncomplete fission-zone formation with arrest in late-cytokinesis, with the new oral apparatus often abnormal and frequently wedged within the arrested furrow.

Expressed in many cells at normal growth temperatures, near 100% at 39°. Same phenotype as the allelic IA137 stock,but with stronger expression.

Submitted by Joseph Frankel, University of Iowa
Research Resource IdentifierTSC_SD01458
Requires MTA?No
Academic cost $35