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Stock IDSD01455
Strain NameIA443
AKAhpo1-3 IIa
SpeciesT. thermophila
Genetic Descriptionhpo1-3/hpo1-3 (hpo1-3; II)
MAC Knockins/outs
MIC Knockins/outs
Gene of InterestHPO1 (hypoangular)
OriginBoth janus and hypoangular clones emerged as F2 progeny following an outcross to B2086 VIa of a mutant clone that resulted from nitrosoguanidine mutagenesis in 1992 of IA264 followed by cytogamy. The hpo-appearing clones were carried through a second outcross to B2086 VIa followed by a genomic-exclusion cross, which yielded some hpo1/hpo1 homozygotes including IA443.
PublicationsBriefly described in Frankel J, Jenkins LM, Nelsen EM, Stoltzman CA. 1993. Hypoangular: A gene potentially involved in specifying positional information in a ciliate, tetrahymena thermophila. Dev Biol 160(2):333-54. (very similar to the more extensively described hpo1-2 allele)
CommentsComplex phenotype with contractile vacuole pores too close to the oral apparatus, and clockwise slippage of oral primordia. The phenotype becomes more extreme (with misshapen cells) after prolonged incubation at elevated temperatures. Allelic to hpo1-1 (IA466)

Submitted by Joseph Frankel, University of Iowa
Research Resource IdentifierTSC_SD01455
Requires MTA?No
Academic cost $35