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Stock IDSD01443
Strain NameIA481
AKAhpo1-4 IV
SpeciesT. thermophila
Genetic Descriptionhpo1-4/hpo1-4 (hpo1-4; IV)
MAC Knockins/outs
MIC Knockins/outs
Gene of InterestHPO1 (hypoangular)
OriginEnigmatic. It arose from a screen designed to detect revertants of janC1. Following nitrosoguanidine mutagenesis of a janC1 heterokaryon in 1994 and uniparental cytogamy, a mutant homozygote was outcrossed to B2086 VIb and then carried through genomic exclusion. The mutant progeny, including IA480 and IA481, all expressed the hypoangular phenotype and showed no trace of the janus phenotype.
PublicationsNone. Very similar to hpo1-1, described in Frankel J, Jenkins LM, Nelsen EM, Stoltzman CA. 1993. Hypoangular: A gene potentially involved in specifying positional information in a ciliate, tetrahymena thermophila. Dev Biol 160(2):333-54.
CommentsA complex phenotype with contractile vacuole pores too close to the oral apparatus, and clockwise slippage of oral primordia. Similar to hpo1-1, though much less extensively studied. A sister clone to IA480.

Submitted by Joseph Frankel, University of Iowa
Research Resource IdentifierTSC_SD01443
Requires MTA?No
Academic cost $35