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Stock IDSD01419
Strain NameTTMN CU428
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (pm-r, VII)
MAC Knockins/outsNeo coding replaces MTT1 coding of some of MTT1 copies, both present
MIC Knockins/outs
Gene of InterestMTT1 (TTHERM_00241640)
OriginYuhua Shang
PublicationsShang Y, Song X, Bowen J, Corstanje R, Gao Y, Gaertig J, Gorovsky MA. 2002. A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in tetrahymena thermophila. Proc Natl Acad Sci U S A 99(6):3734-9.
CommentsCU428 transformed by pTTMN construct and cells selected with 10 mg/ml paromomycin. Partially replaced. Still has wt Metallothionein gene copies in mac as well as other alleles with neo coding.
Research Resource IdentifierTSC_SD01419
Requires MTA?No
Academic cost $35