Stock ID | SD01363 |
Strain Name | T146VHA (a) |
AKA | |
Species | T. thermophila |
Genetic Description | gtu1[∆::neo2]/gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, HAc(::GTU1, T146V)]; pm-r, ?) |
MAC Knockins/outs | GTU1 with mutation T146V and c-terminus HA tag into the MTT1 locus, partial replacement. |
MIC Knockins/outs | Neo2 into GTU1 locus |
Gene of Interest | GTU1 (TTHERM_00079520) |
Origin | Yuhua Shang |
Publications | Shang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206. Shang Y, Tsao CC, Gorovsky MA. 2005. Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. J Cell Biol 171(6):1035-44. |
Comments | pT146V-HA rescued GTUKO 5x6 progeny having mutation @ GTU1 T146V gene, driven by MTT1 promoter. Has HA tag at c-terminus. Single cell (a) Grow and maintain in 0.5 ug/ml CdCl2 at 30C. From the Gorovsky lab, University of Rochester. |
Research Resource Identifier | TSC_SD01363 |
Requires MTA? | No |
Academic cost | $45 |