Stock ID | SD01317 |
Strain Name | ∆H3/H4, major, gl KO 7(19) |
AKA | |
Species | T. thermophila |
Genetic Description | hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2];hhf1[neo1]/hhf1[neo1]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?) |
MAC Knockins/outs | |
MIC Knockins/outs | Neo2 into HHT1 and KO HHT2/HHF2 gene and its intergenic region; KO of HHF1 |
Gene of Interest | HHT + HHF |
Origin | Yifan Liu |
Publications | Cui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30. Liu Y, Song X, Gorovsky MA, Karrer KM. 2005. Elimination of foreign DNA during somatic differentiation in tetrahymena thermophila shows position effect and is dosage dependent. Eukaryot Cell 4(2):421-31. |
Comments | This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2. KO with Neo cassette. HHF1 KO. It’s been tested to cross with ∆H3/H4 major,4(1) and the progeny died. Somatic rescue successful From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
Research Resource Identifier | TSC_SD01317 |
Requires MTA? | No |
Academic cost | $45 |