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Stock IDSD00636
Strain NameIA344
AKAbcd1-1 VII
SpeciesT. thermophila
Genetic Descriptionbcd1-1/bcd1-1; eja1-1/eja1-1 (bcd1-1; eja1-1; bcd1, eja1, VII)
MAC Knockins/outs
MIC Knockins/outs
Gene of InterestBCD1 (broadened cortical domains); EJA1 (enhancer of janus-A)
OriginGenomic exclusion progeny following two successive generations of outcrossing to IA264 of a mutant clone resulting from nitrosoguanidine mutagenesis of IA264 in 1984 followed by cytogamy. Sister clone to IA342.
PublicationsGene:
Cole ES, Frankel J, Jenkins LM. 1987. Bcd: A mutation affecting the width of organelle domains in the cortex of tetrahymena thermophila. Development Genes and Evolution 196(7):421-33.

This clone:
Cole ES, Frankel J, Jenkins LM. 1988. Interactions between janus and bcd cortical pattern mutants in tetrahymena thermophila. Development Genes and Evolution 197(8):476-89.

Cole ES. 1991. Conjugal blocks in tetrahymena pattern mutants and their cytoplasmic rescue. I. broadened cortical domains (bcd). Dev Biol 148(2):403-19.
CommentsThe phenotype of this clone is very similar to that of IA342. Exhibits conjugal block, primarily due to failure of nuclear fusion.
Research Resource IdentifierTSC_SD00636
Requires MTA?No
Academic cost $45