Stock ID | SD00636 |
Strain Name | IA344 |
AKA | bcd1-1 VII |
Species | T. thermophila |
Genetic Description | bcd1-1/bcd1-1; eja1-1/eja1-1 (bcd1-1; eja1-1; bcd1, eja1, VII) |
MAC Knockins/outs | |
MIC Knockins/outs | |
Gene of Interest | BCD1 (broadened cortical domains); EJA1 (enhancer of janus-A) |
Origin | Genomic exclusion progeny following two successive generations of outcrossing to IA264 of a mutant clone resulting from nitrosoguanidine mutagenesis of IA264 in 1984 followed by cytogamy. Sister clone to IA342. |
Publications | Gene: Cole ES, Frankel J, Jenkins LM. 1987. Bcd: A mutation affecting the width of organelle domains in the cortex of tetrahymena thermophila. Development Genes and Evolution 196(7):421-33. This clone: Cole ES, Frankel J, Jenkins LM. 1988. Interactions between janus and bcd cortical pattern mutants in tetrahymena thermophila. Development Genes and Evolution 197(8):476-89. Cole ES. 1991. Conjugal blocks in tetrahymena pattern mutants and their cytoplasmic rescue. I. broadened cortical domains (bcd). Dev Biol 148(2):403-19. |
Comments | The phenotype of this clone is very similar to that of IA342. Exhibits conjugal block, primarily due to failure of nuclear fusion. |
Research Resource Identifier | TSC_SD00636 |
Requires MTA? | No |
Academic cost | $45 |