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Stock IDSD00630
Strain NameIA220
AKAjanA-1 II
SpeciesT. thermophila
Genetic DescriptionjanA-1/janA-1; eja1-1/eja1-1; chx1-1/chx1-1 (janA-1; eja1-1; chx1-1; janA, eja1, cy-r, II)
MAC Knockins/outs
MIC Knockins/outs
Gene of InterestJANA (janus A) (originally reported as jan); EJA1 (enhancer of janus A) (originally reported as eja, "enhancer of janus").
OriginThe original janA clone was obtained by genetic rescue of a clone selected in 1976 by Bruns and Sanford (PNAS 75: 3355-3358) using nitrosoguanidine mutagenesis followed by short-circuit genomic exclusion. The derivation of IA220 included four generations of outcrossing of the original janA clone , the first two described by Frankel & Jenkins (1979) and the latter two by Frankel et al. (1984), which also describes the discovery and verification of eja1-1.
PublicationsGenes:
Frankel J and Jenkins LM. 1979. A mutant of tetrahymena thermophila with a partial mirror-image duplication of cell surface pattern. II. nature of genic control. J Embryol Exp Morphol 49:203-27.

Frankel J, Jenkins LM, Bakowska J. 1984. Selective mirror-image reversal of ciliary patterns intetrahymena thermophila homozygous for ajanus mutation. Development Genes and Evolution 194(2):107-20.

This clone:
Strzyzewska-Jowko I, Jerka-Dziadosz M, Frankel J. 2003. Effect of alteration in the global body plan on the deployment of morphogenesis-related protein epitopes labeled by the monoclonal antibody 12G9 in tetrahymena thermophila. Protist 154(1):71-90.

Cole ES and Frankel J. 1991. Conjugal blocks in tetrahymena pattern mutants and their cytoplasmic rescue. II. janus A. Dev Biol 148(2):420-8.

Several other references as well (see: Frankel J. 2008. What do genic mutations tell us about the structural patterning of a complex single-celled organism? Eukaryot Cell 7(10):1617-39.)
CommentsA mutation that causes the dorsal cell surface to be converted to a partial mirror-image of the ventral surface and also brings about conjugal blockage. eja1-1 is referred to as "eja" in publications starting with Frankel et al. (1984) R.A. Dev. Biol. 194: 107-120, except where it was mistakenly designated as "enj" in some papers published after 1984.
Research Resource IdentifierTSC_SD00630
Requires MTA?No
Academic cost $45